Amounts used in published tests
TB-500 is a short protein piece with no proven human dose.
This page lists amounts from set studies. Some came from animals; others concerned the full protein. They don't direct your use.
Study amounts ranged from tiny drops to large vein doses
Most TB-500 amount claims come from animal tests of full thymosin beta-4. Rat heart and brain work often used about 6 to 12 milligrams per kilogram of weight. In the stroke test, belly shots held 2 milligrams per kilogram, 12 milligrams per kilogram, or 18 milligrams per kilogram. A math estimate favored about 3.75 milligrams per kilogram [4]. Another test gave mice 150 micrograms two times a week across six months. Those mice had a disease that weakens muscle, and they also got belly shots [5].
The one test in healthy adults was Phase 1, the earliest safety step. It gave set amounts of full thymosin beta-4. Healthy adults received 42, 140, 420, or 1260 mg by vein. They first received one amount; another came each day for 14 days. The adults handled the largest amount during that short test [6]. At the other end, about 10 picograms moved skin cells in a lab dish [3]. It takes a trillion picograms to make one gram.
You can't turn these numbers into directions for TB-500. Each amount belongs to its own test, and none gave people the small piece. The TB-500 study findings let you see every amount beside its study.
Animals got belly shots; people got the full protein by vein
Rat and mouse studies usually gave shots into the belly [4][5]. In Phase 1, the first human safety stage sent full thymosin beta-4 through a vein. Some heart tests also used veins [6]. Skin and eye studies applied the protein straight to the sore area. RGN-259 was given as a dry-eye drop [7]. Users describe shots into muscle or the fat under skin. Careful human trials have not found that either way works [5].
The way a medicine enters your body can change its effect. Phase 1 safety came from vein use, while wound gains followed skin use or belly shots. A result from one way cannot simply cover another. FDA says some ways could make the immune system react against the drug. The public note doesn't name those ways. The TB-500 legal status and 503A one-patient pharmacy page explains what FDA published.
The TB-500 half-life in people is not known
No sound study tells you how long TB-500 stays active in a person [5]. Doctors call that span a half-life. A number borrowed from another test cannot fill the gap.
The nearest human facts still concern the full protein. During the Phase 1 vein study, the first safety stage, larger amounts remained longer in the body [6]. There was no single fixed time. Drug testers detected the fragment and broken-down parts in samples of horse blood and urine [15]. Their test was meant to catch TB-500 in sport, not time it in people.
Users call a larger plan at the start "loading." They call the later ongoing plan "maintenance." No fair human trial backs either plan [5]. The rat stroke result also warns against guesswork. The amount of 18 milligrams per kilogram did worse than 12 milligrams per kilogram [4]. More, or more often, isn't a safe guess for you.
Research powder can break down, and its contents may vary
Research sellers offer TB-500 as freeze-dried powder [5]. Their directions use germ-free water and cold storage. The fragment might remain whole longer than the full protein, yet body chemicals can still cut it apart. Freezing and thawing it more than once can also damage the material.
A research label cannot prove the contents of a vial. With no drug rules guarding the supply, another substance or unwanted matter may be inside. A vial could even contain the full protein while its label names the short piece [5]. That doubt makes the user stories difficult for you to weigh. A fair study deserves greater trust.